PYY (3-36)-Gly (Canine, Rat, Mouse, Porcine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human Colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (3-36)-Gly (R,M,C,P) Antiserum (Catalog No.: H-059-09)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-059-09

Standard Size

100 µl

Species

Rat, Mouse, Porcine, Canine

Host

Rabbit

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.