Augurin, Prepro (133-148) (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry
Tissue Sample	Human pancreas tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Augurin, Prepro (133-148) (H) Antiserum (Catalog No.: H-012-24)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Lane #1: Porcine Pancreas Extracts
Lane #2: Bovine Hypothalamus Extracts
Lane #3: Bovine Pituitary Extracts
Lane #4: Porcine Liver Extracts
The major band in the porcine pancreas is 14.2 kDa augurin, but the bovine hypothalamus and pituitary show augurin derived peptides with the size at 14.1, 9.6 kDa and 6.5 kDa which can be detected by anti-augurin (133-148) antibody.

Catalog #

H-012-24

Family

augurin

Standard Size

100 µl

Species

Human

Host

rabbit

Immunogen

augurin, prepro (133-148) (H)

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry - 1:500

Alphabet_A_