Chemerin / TIG2 (145-157) (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample	Human Hepatoma tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-TIG2 /Chemerin (145-157) (H) Antiserum (Catalog No.: H-002-50)
Optimal Dilution	1:500 (1 hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-50

Standard Size

100 µl

Species

Human

Specific Specie

Rabbit

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry - 1:500

Alphabet_C_

Please click here or click the reference tab on the top to see more articles that use this product.