Neuropeptide S (1-10) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human Brain Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum, 3%H₂O₂
Primary Antibody	Rabbit anti-Neuropeptide S (NPS) (1-10) Serum (Catalog No.: H-005-81)
Optimal Dilution	1:500 (1 hour at RT )
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification	ABC (Vector)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin

Catalog #

H-005-81

Family

NPS

Standard Size

100 ul

Sequence

Ser - Phe - Arg - Asn - Gly - Val - Gly - Thr - Gly - Met

Species

Human

Host

Rabbit

Immunogen

human NPS (1-10)

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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