Alarin (Rat) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Mouse/Rat Brain Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Alarin (Rat) Antibody (Catalog No.: H-026-33)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-026-33

Family

Alarin

Standard Size

100 µl

Sequence

Ala - Pro - Ala - His - Arg - Ser - Ser - Thr - Phe - Pro - Gln - Arg - Pro - Thr - Arg - Ala - Gly - Arg - Gln - Thr - Gln - Leu - Leu - Arg - Ser

Species

Rat

Host

Rabbit

Immunogen

Alarin (Rat)

Reconstitution

100 µl

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry- 1:500

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