INSL 6 C-Peptide, Prepro (55-118) (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample	Rat and mouse testis
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-INSL6, C Peptide (Human) Antibody (Catalog No.:H-035-57)
Optimal Dilution	1: 200
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-035-57

Family

INSL

Standard Size

100 µl

Sequence

Phe - Glu - Glu - Glu - Thr - Pro - Phe - Ser - Arg - Leu - Ile - Ala - Gln - Ala - Ser - Glu - Lys - Val - Glu - Ala - Tyr - Ser - Pro - Tyr - Gln - Phe - Glu - Ser - Pro - Gln - Thr - Ala - Ser - Pro - Ala - Arg - Gly - Arg - Gly - Thr - Asn - Pro - Val - Ser - Thr - Ser - Trp - Glu - Glu - Ala - Val - Asn - Ser - Trp - Glu - Met - Gln - Ser - Leu - Pro - Glu - Tyr - Lys - Asp

Species

Human

Host

Rabbit

Immunogen

Human INSL6, C Peptide

Reconstitution

100 ul

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_C_