Catestatin Variant (Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human pancreas tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Catestatin (Mouse) Antibody (Cat. No.: H-053-28)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (sigma), 30min
Counterstained	Hematoxylin, 30Sec

Catalog #

H-053-28

Standard Size

100 µl

Sequence

Arg - Ser - Met - Arg - Leu - Ser - Phe - Arg - Thr - Arg - Gly - Tyr - Gly - Phe - Arg - Asp - Pro - Gly - Leu - Gln - Leu

Species

Mouse

Host

Rabbit

Immunogen

Catestatin (Mouse)

Reconstitution

100 ul

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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