Adrenomedullin (1-44) (Human) - Antibody for Immunohistochemistry

Image

H-010-28-2444-3-H-500 H-010-28-2444-3-R-500-lung

Protocol for Immunohistochemistry
Tissue Sample	Human/Rat Lung and Lunch Cancer Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No Primary Antibody
Pretreatment	Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum, 3% H₂O₂
Primary Antibody	Anti-Adrenomedullin (1-44) (Human) Antibody (Catalog No.: H-010-28)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-010-28

Family

Adrenomedullin

Standard Size

100 µl

Sequence

Tyr - Arg - Gln - Ser - Met - Asn - Asn - Phe - Gln - Gly - Leu - Arg - Ser - Phe - Gly - Cys - Arg - Phe - Gly - Thr - Cys - Thr - Val - Gln - Lys - Leu - Ala - His - Gln - Ile - Tyr - Gln - Phe - Thr - Asp - Lys - Asp - Lys - Asp - Asn - Val - Ala - Pro - Arg, Disulfide Bridge: Cys16 - Cys21

Species

Human

Host

Rabbit

Immunogen

Adrenomedullin (1-44) (Human)

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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