C-Peptide 1 (Rat) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human pancreas tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-C Peptide-1 (Rat) Antiserum (Catalog No.: H-035-03)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-035-03

Family

Insulin

Standard Size

100 ul

Sequence

Glu - Val - Glu - Asp - Pro - Gln - Val - Pro - Gln - Leu - Glu - Leu - Gly - Gly - Gly - Pro - Glu - Ala - Gly - Asp - Leu - Gln - Thr - Leu - Ala - Leu - Glu - Val - Ala - Arg - Gln

Species

Rat

Host

Rabbit

Immunogen

C Peptide, Insulin-1 (Rat)

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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