Adrenomedullin-Gly (Human) - Antibody for Immunohistochemistry

Image

H-010-37-2429-3-H-500 H-010-37-2430-3-H-500

Protocol for Immunohistochemistry
Tissue Sample	Human Lung Cancer & Pancreatic Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No Primary Antibody
Pretreatment	Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum, 3% H₂O₂
Primary Antibody	Anti-Adrenomedullin-Gly (Human) Antibody (Catalog No.: H-010-37)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-010-37

Family

Adrenomedullin

Standard Size

100 µl

Sequence

Tyr - Arg - Gln - Ser - Met - Asn - Asn - Phe - Gln - Gly - Leu - Arg - Ser - Phe - Gly - Cys - Arg - Phe - Gly - Thr - Cys - Thr - Val - Gln - Lys - Leu - Ala - His - Gln - Ile - Tyr - Gln - Phe - Thr - Asp - Lys - Asp - Lys - Asp - Asn - Val - Ala - Pro - Arg - Ser - Lys - Ile - Ser - Pro - Gln - Gly - Tyr - Gly Disulfide Bridge: Cys16 - Cys21

Species

Human

Host

Rabbit

Immunogen

Adrenomedullin-Gly

Reconstitution

For best and reproducible results, reconstitute with 100 µl of distilled water for the equivalent of undiluted antiserum. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Appearance

lyophilized form.

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