Cathepsin S (298-331) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Mouse & rat visceral fat tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Cathepsin S (298-331) (Human) Serum (Catalog No.:H-002-82)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-82

Standard Size

50 µl

Sequence

Please call for status of this Item

Species

Human

Reconstitution

Reconstitute with 100 µl distill water.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Cross Reactivity

This antibody crossreacts with human Cathepsin S (298-331) at 100%. It was tested in immunohistochemistry using mouse and rat visceral fat tissue..

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