NPFF-2R (291-308) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat & Mouse Brain
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-NPFF2R (291-308) (H) Serum (Catalog No.:H-001-59)
Optimal Dilution	1:200 (1 hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-001-59

Standard Size

100 µl

Sequence

Tyr - Tyr - Arg - Val - Arg - Leu - Asn - Ser - Gln - Asn - Lys - Thr - Ser - Pro - Val - Tyr - Trp - Cys

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100ul of distilled water for the equivalent of undiluted antiserum, immediately before use. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100ul of rabbit anti- NPFF-2R (291-308) (H) serum in the lyophilized form.

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