LEAP-2 (38-77) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Rat/mouse kidney & rat liver
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody (pre-immuno serum)
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-LEAP-2 (38-77) (Human) Serum (Catalog No.:H-075-40)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-075-40

Standard Size

100 µl

Species

Human

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to the publications that use this antibody:

Howard A, Townes C, Milona P, Nile CJ, Michailidis G, Hall J. Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues.
Cell Immunol. 2010;261(2):128-33.