EM-66 / Secretogranin II (219-284) (Rat) - Antibody for Immunohistochemistry

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Western Blot Analysis of Secretogranin II in rat brain tissue
Tissue Sample	Rat brain tissue
Electrophoresis	10% SDS-PAGE gel, reducing condition
Denatured sample	100'C for 5 min
Positive control	Rat Secretogranin II peptide
Blocking	1% BSA in TBST
Primary Antibody	Rabbit Anti-EM-66/Secretogranin II (219-284) (Rat) Serum (Catalog No.:H-047-69)
Optimal Dilution	1:200 (overnight at cold room)
Secondary Antibody	Anti-Rabbit whole IgG, HRP conjugated
Positive Band	~ 68 KDa
Substrate	ECL

Catalog #

H-047-69

Standard Size

100 µl

Sequence

Glu - Arg - Val - Asp - Glu - Glu - Gln - Lys - Leu - Tyr - Thr - Asp - Asp - Glu - Asp - Asp - Val - Tyr - Lys - Thr - Asn - Asn - Ile - Ala - Tyr - Glu - Asp - Val - Val - Gly - Gly - Glu - Asp - Trp - Ser - Pro - Met - Glu - Glu - Lys - Ile - Glu - Thr - Gln - Thr - Gln - Glu - Glu - Val - Arg - Asp - Ser - Lys - Glu - Asn - Thr - Glu - Lys - Asn - Glu - Gln - Ile - Asn - Glu - Glu - Met

Species

Rat

Host

Rabbit

Reconstitution

Reconstitute with 100 µl distill water.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Direct ELISA: 1: 3200
Western blot : 1:200

Cross Reactivity

This antibody is crossreactivity with rat EM-66 peptide or Secretogranin II (219-284) at 100%. It was tested in western blot in rat brain tissues.

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