Oxyntomodulin (Porcine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Mouse or Rat Pancreas tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Oxyntomodulin (Porcine) Serum (Catalog No.:H-028-16)
Optimal Dilution	1:250~500 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-028-16

Standard Size

100 µl

Sequence

His - Ser - Gln - Gly - Thr - Phe - Thr - Ser - Asp - Tyr - Ser - Lys - Tyr - Leu - Asp - Ser - Arg - Arg - Ala - Gln - Asp - Phe - Val - Gln - Trp - Leu - Met - Asn - Thr - Lys - Arg - Asn - Lys - Asn - Asn - Ile - Ala

Species

Porcine

Reconstitution

Reconstitute with 100 µl distill water.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Direct ELISA: 1: 6400
Immunohistochemistry (ABC): 1: 250~500

Cross Reactivity

This antibody is crossreactive with porcine Oxyntomodulin. It also works for rat/mouse tissue immunohistochemistry.

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