Angiopoietin-related Growth Factor (AGF) / Angiopoietin-like 6 (215-230) (Mouse)

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Protocol for Immunohistochemistry
Tissue Sample	Rat liver Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-AGF/ANGPTL6 (215-350) (Mouse) serum (Catalog No.: H-028-92)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-028-92

Standard Size

100 µl

Species

Mouse

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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