Musclin / Osteocrin (Ostn) (113-130) (Mouse) / (115-132) (Rat)

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Protocol for Immunohistochemistry:
Tissue Sample	Rat heart tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Target retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum
Primary Antibody	Musclin (113-130)(Mouse) / Musclin (115-132) (Rat) serum (Catalog No.:H-028-65)
Optimal Dilution	1:50 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector)(1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-028-65

Standard Size

50 µl

Species

Rat, Mouse

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_M, O_