Brain Natriuretic Peptides (BNP)

Normal Plasma Peptide/Protein Levels

Antibody Production & Evaluation

Catalog #	H-011-03
Standard Size	50 µl
Sequence	Ser - Pro - Lys - Met - Val - Gln - Gly - Ser - Gly - Cys - Phe - Gly - Arg - Lys - Met - Asp - Arg - Ile - Ser - Ser - Ser - Ser - Gly - Leu - Gly - Cys - Lys - Val - Leu - Arg - Arg - His Disulfide Bridge: Cys10 - Cys26
Species	Human
Host	Rabbit
Reconstitution	For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition	Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution Factor	Immunofluorescence: 1:200 PAP or ABC: 1:500
Cross Reactivity	Peptide  % Cross-reactivity  BNP-32 (Human)  100 BNP-32 (Rat)  <0.04 BNP-45 (Rat  0 BNP-26 (Porcine) 0  α-ANP (1-28) (Human, Canine)   0  Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)  0  Angiotensin II (Human)  0  VIP (Human, Rat, Porcine)  0

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Links to publications that use this product:

Herten et al. The renal natriuretic peptide urodilatin is present in human kidney.
Nephrol Dial Transplant. 1998 Oct;13(10):2529-35.