GPR-39 (423-453) (Human) - Antibody for Immunohistochemistry

Image

Western Blot Analysis
Sample	Mouse and rat brain tissue homogenate
Electrophoresis	10% SDS-PAGE gel, reduce condition
Negative control	100^oC for 5 min
Denatured sample	1% BSA in TBST
Primary Antibody	Rabbit Anti-GPR 39 (423-453) (Human) Antibody (Catalog No.:H-002-85)
Optimal Dilution	1: 500 (overnight at 4 ^oC)
Secondary Antibody	Rabbit anti-rabbit IgG, HRP conjugated
Substrate	ECL
Positive Band	~ 50 KDa

Protocol for Immunohistochemistry:
Tissue Sample	Rat & Mouse brain tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-GPR 39 (423-453) (Human) Antiserum (Catalog No.:H-002-85)
Optimal Dilution	1:500 (overnight at 4 ^oC)
Secondary Antibody	Goat anti-Rabbit IgG, HRP conjugated
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-85

Standard Size

100 µl

Species

Human

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_G_

Please click here or click the reference tab on the top to see more articles that use this product.