GPR-30 (1-23) [Cys24] (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample	Mouse & rat brain tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-GPR30 (1-23) (Human) Serum (Catalog No.:H-002-83)
Optimal Dilution	1:500
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-83

Standard Size

100 µl

Species

Human

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_G_