Adrenomedullin N-20, Pro / PAMP-20 / Prodepin (Rat) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Rat Lung Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No Primary Antibody
Pretreatment	Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum, 3% H₂O₂
Primary Antibody	Anti-PAMP-20 (rat) Antibody (Catalog No.: H-010-10)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-010-10

Family

Adrenomedullin

Standard Size

100 µl

Sequence

Ala - Arg - Leu - Asp - Thr - Ser - Ser - Gln - Phe - Arg - Lys - Lys - Trp - Asn - Lys - Trp - Ala - Leu - Ser - Arg - NH2

Species

Rat

Host

Rabbit

Immunogen

Adrenomedullin N-20, Pro/PAMP-20 (Rat)

Reconstitution

For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of Rabbit Anti-Pro-Adrenomedullin N-20 (Rat) Serum in the lyophilized form.

Recommended Dilution Factor

ABC- 1:500
Immunohistochemistry- 1:200
Immunohistochemistry- 1:500

Cross Reactivity

Peptide % Cross-reactivity
Pro-Adrenomedullin N-20 (Rat) 100

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that used this product:

Cameron et al. Adrenomedullin expression in rat uterus is correlated with plasma estradiol
Am J Physiol Endocrinol Metab. 2002 Jan;282(1):E139-46.

Qi et al. Chronic administration of adrenomedullin attenuates hypoxic pulmonary vascular structural remodeling and inhibits proadrenomedullin N-terminal 20-peptide production in rats
Peptides. 2007 Apr;28(4):910-9.