Amylin (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample Human Pancreas tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Target retrieval 10 min (Steam)*
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Amylin (Human) Purified IgG (Catalog No:H-017-03)
Optimal Dilution 1:200 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec
* Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Catalog #

H-017-03

Standard Size

50 µl

Sequence

Lys - Cys - Asn - Thr - Ala - Thr - Cys - Ala - Thr - Gln - Arg - Leu - Ala - Asn - Phe - Leu - Val - His - Ser - Ser - Asn - Asn - Phe - Gly - Ala - Ile - Leu - Ser - Ser - Thr - Asn - Val - Gly - Ser - Asn - Thr - Tyr - NH2

Species

Human

Host

Rabbit

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-Amylin (Human) Antibody Serum in the lyophilized form.

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