Bradykinin (Human, Rat, Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Rat Spinal Cord
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No Primary Antibody
Pretreatment	Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Bradykinin Antiserum (Catalog No.:H-009-01)
Optimal Dilution	1:200 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-009-01

Standard Size

50 µl

Sequence

Arg - Pro - Pro - Gly - Phe - Ser - Pro - Phe - Arg

Species

Human, Rat, Mouse

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit Anti-Bradykinin Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:200
Immunohistochemistry: 1:500

Cross Reactivity

*Peptide*	*% Cross-reactivity*
Bradykinin	100
Lys-Bradykinin	100
Biotinyl-Bradykinin	100
[Tyr0]-Bradykinin	100
[Des-Arg9]-Bradykinin	0
Met-Enkephalin	0
Substance P	0
Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)	0
TRH	0

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