Corticotropin Releasing Factor (CRF) (Human, Rat, Mouse, Canine, Feline)

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Corticotropin Releasing Factor (CRF) (Human, Rat, Mouse, Canine, Feline) - Antibody for Immunohistochemistry

Catalog#	Standard size	Price	Order
H-019-06	50 µl	$175.00

Image

Protocol for Immunohistochemistry:
Tissue Sample Rat hypothalamus
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Target Retrieval 25 min (Steam)
Blocking 2% Normal Goat Serum
Primary Antibody Anti-Corticotropin Releasing Factor (CRF) (Human, Rat) Serum
Optimal Dilution 1:100
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification ABC (Vector)
Detection system HRP
Substrate DAB (Sigma)
Counterstained Hematoxylin

Catalog #

H-019-06

Standard Size

50 µl

Sequence

Ser - Glu - Glu - Pro - Pro - Ile - Ser - Leu - Asp - Leu - Thr - Phe - His - Leu - Leu - Arg - Glu - Val - Leu - Glu - Met - Ala - Arg - Ala - Glu - Gln - Leu - Ala - Gln - Gln - Ala - His - Ser - Asn - Arg - Lys - Leu - Met - Glu - Ile - Ile - NH2

Species

Human, Rat, Mouse

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-Corticotropin Releasing Factor (Human, Rat) Serum in the lyophilized form.

Cross Reactivity

*Peptide*	*%Cross-reactivity*
CRF (Human, Rat)	100
ACTH (Human)	0
LH-RH	0
PACAP-38 (Human, Rat, Ovine)	0
[Arg⁸]-Vasopressin	0
Urocortin (Human)	0
Urocortin (Rat)	0
BNP-45 (Rat)	0

Alphabet_C_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Vergetaki A, Jeschke U, Vrekoussis T, et al. Differential Expression of CRH, UCN, CRHR1 and CRHR2 in Eutopic and Ectopic Endometrium of Women with Endometriosis.
PLoS ONE. 2013;8(4):e62313-.

Eun Hyun Kim et al., The expression of corticotropin-releasing factor and its receptors in the spinal cord and dorsal root ganglion in a rat model of neuropathic pain.
Anat Cell Biol 2011;44:60-68.

LaBerge et al. Postnatal expression of corticotropin releasing factor (CRF) in rat urinary bladder.
Auton Neurosci. 2008 Aug 18;141(1-2):83-93.

He et al. Corticotropin-releasing hormone receptor 1 coexists with endothelin-1 and modulates its mRNA expression and release in rat paraventricular nucleus during hypoxia.
Neuroscience. 2008 Apr 9;152(4):1006-14.

Kim et al. Expression of the corticotropin-releasing hormone–proopiomelanocortin axis in the various clinical types of psoriasis
Exp Dermatol. 2007 Feb;16(2):104-9.

Minas et al. Intratumoral CRH modulates immuno-escape of ovarian cancer cells through FasL regulation
Br J Cancer. 2007 Sep 3;97(5):637-45.

Heisler et al. Serotonin Activates the Hypothalamic–Pituitary–Adrenal Axis via Serotonin 2C Receptor Stimulation
J Neurosci. 2007 Jun 27;27(26):6956-64.

Kim et al. Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours.
Br J Dermatol. 2006 Nov;155(5):910-5.

Kim et al. Immunoreactivity of corticotropin-releasing hormone, adrenocorticotropic hormone and alpha-melanocyte-stimulating hormone in alopecia areata.
Exp Dermatol. 2006 Jul;15(7):515-22.

Charalampopoulos et al. Neuropeptide urocortin and its receptors are expressed in rat Kupffer cells.
Neuroendocrinology. 2006;84(1):49-57.

Studeny et al. Corticotropin-releasing factor (CRF) expression in postnatal and adult rat sacral parasympathetic nucleus (SPN).
Cell Tissue Res. 2005 Dec;322(3):339-52.

Xu et al. CRF receptor type 1 mediates continual hypoxia-induced CRF peptide and CRF mRNA expression increase in hypothalamic PVN of rats.
Peptides. 2005 Apr;26(4):639-46.

Chatzaki et al. Urocortin and corticotropin-releasing hormone receptor type 2 expression in the human gallbladder.
Neuroendocrinology. 2005;82(3-4):177-84.

Kempuraj et al. Increased numbers of activated mast cells in endometriosis lesions positive for corticotropin-releasing hormone and urocortin.
Am J Reprod Immunol. 2004 Oct;52(4):267-75.

Sari et al. Effect of prolonged exposure to low concentrations of formaldehyde on the corticotropin releasing hormone neurons in the hypothalamus and adrenocorticotropic hormone cells in the pituitary gland in female mice.
Brain Res. 2004 Jul 2;1013(1):107-16.

Kempuraj et al. Corticotropin-releasing hormone and its structurally related urocortin are synthesized and secreted by human mast cells.
Endocrinology. 2004 Jan;145(1):43-8.