Urocortin (Rat, Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Rat Piriform Cortex
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Urocortin (Rat) (Human) Antibody (Catalog No.: H-019-15)
Optimal Dilution	1:100
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-019-15

Standard Size

50 µl

Sequence

Asp - Asp - Pro - Pro - Leu - Ser - Ile - Asp - Leu - Thr - Phe - His - Leu - Leu - Arg - Thr - Leu - Leu - Glu - Leu - Ala - Arg - Thr - Gln - Ser - Gln - Arg - Glu - Arg - Ala - Glu - Gln - Asn - Arg - Ile - Ile - Phe - Asp - Ser - Val - NH2

Species

Rat, Mouse

Host

Rabbit

Immunogen

Urocortin (Rat)

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-Urocortin (Rat) Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

Cross Reactivity

Peptide	*% Cross-reactivity*
Urocortin (Rat, Mouse)	100
Urocortin (Human)	100
Cst-14 (Rat)	1.94
CRF (Human, Rat)	0.0015
MCH (Human, Mouse, Rat)	0
LH-RH (Human, Porcine, Rat)	0
Somatostatin	0
NPY (Human, Rat)	0

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Laursen et al. The Edinger-Westphal nucleus of the juvenile rat contains transient- and repetitive-firing neurons.
Neuroscience. 2006 Aug 11;141(1):191-200.

Charalampopoulos et al. Neuropeptide urocortin and its receptors are expressed in rat Kupffer cells.
Neuroendocrinology. 2006;84(1):49-57.