ANP (56-92), Prepro (Human) - Antibody for Immunohistochemistry

Image

Immunoperoxidase localization of vessel dilator [Rabbit Anti-Atrial Natriuretic Peptide (ANP) (56-92)-Prepro (Human) Antibody, Cat.No. H-005-20]within the kidney demonstrating staining through out the kidney but of increased intensity of staining in the proximal tubules (T) of this normal rat kidney. Original magnification x60. Primary antibody diluted 1:800.

Protocol for Immunohistochemistry:
Tissue Sample	Rat kidney
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Atrial Natriuretic Peptide (ANP) (56-92)-Prepro (Human) Antibody, Cat.No. H-005-20
Optimal Dilution	1:800
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (sigma), 30min
Counterstained	Hematoxylin, 30Sec

Catalog #

H-005-20

Standard Size

50 µl

Sequence

Glu - Val - Val - Pro - Pro - Gln - Val - Leu - Ser - Glu - Pro - Asn - Glu - Glu - Ala - Gly - Ala - Ala - Leu - Ser - Pro - Leu - Pro - Glu - Val - Pro - Pro - Trp - Thr - Gly - Glu - Val - Ser - Pro - Ala - Gln - Arg

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit anti prepro-ANF 56-92 (Human) serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

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