ANP (Atrial Natriuretic Peptide)

Normal Plasma Peptide/Protein Levels

Antibody Production & Evaluation

Image	Protocol for Immunohistochemistry:  Tissue Sample  Rat or Mouse heart tissue  Fixative  10% Formalin  Embedding  Paraffin  Negative control  No primary antibody  Pretreatment  Intact  Blocking  2% Normal Goat Serum  Primary Antibody  Rabbit Anti-ANP-alpha (1-28) (Human) (Catalog No.:H-005-06)  Optimal Dilution  1:500  Secondary Antibody  Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)  Amplification  ABC (Vector) (1:400, 30 min)  Detection system  HRP  Substrate  DAB (sigma), 30min  Counterstained  Hematoxylin, 30Sec
Catalog #	H-005-06
Family	ANP
Standard Size	50 µl
Sequence	Ser - Leu - Arg - Arg - Ser - Ser - Cys - Phe - Gly - Gly - Arg - Met - Asp - Arg - Ile - Gly - Ala - Gln - Ser - Gly - Leu - Gly - Cys - Asn - Ser - Phe - Arg - Tyr Disulfide Bridge: Cys7 - Cys23
Species	Human, Canine, Ovine, Porcine
Host	Rabbit
Reconstitution	For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage Condition	Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content	This vial contains 50µl of rabbit anti- Atrial Natriuretic Polypeptide (a-ANP) (1-28) (Human, Canine, Porcine, Bovine) Serum in the lyophilized form.
Recommended Dilution Factor	Immunofluorescence- 1:200 ABC- 1:500
Cross Reactivity	Peptide  % Cross-reactivity   a-ANP (1-28) (Human, Ovine, Canine)  100  b-ANP (Human  100  Urodilatin  100  ANP (Rat)  0.23  BNP-32 (Human)  0.024  Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)  0.01  CNP (24-53)  0.009  BNP-26 (Porcine)  0.006  BNP-45 (Rat)  0

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Biondo et al. Immunohistochemistry of atrial and brain natriuretic peptides in control cats and cats with hypertrophic cardiomyopathy.
Vet Pathol. 2003 Sep;40(5):501-6.

Bialik et al. Evaluation of atrial natriuretic peptide and brain natriuretic peptide in atrial granules of rats with experimental congestive heart failure.
J Histochem Cytochem. 2001 Oct;49(10):1293-300.

Chen et al. The modulating actions of sulfonylurea on atrial natriuretic peptide release in experimental acute heart failure.
Eur J Heart Fail. 2000 Mar;2(1):33-40.

Lai et al. Up-regulation of adrenal cortical and medullary atrial natriuretic peptide and gene expression in rats with deoxycorticosterone acetate-salt treatment.
Endocrinology. 2000 Jan;141(1):325-32.