Visfatin (400-450) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human visceral white fat tissue, rat & mouse liver tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative Control	No primary antibody (pre-immuno serum)
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Visfatin (400-450) (Human) Antiserum (Catalog No.:H-003-84)
Optimal Dilution	1:200-500 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-003-84

Standard Size

100 µl

Sequence

Lys - Cys - Ser - Tyr - Val - Val - Thr - Asn - Gly - Leu - Gly - Ile - Asn - Val - Phe - Lys - Asp - Pro - Val - Ala - Asp - Pro - Asn - Lys - Arg - Ser - Lys - Lys - Gly - Arg - Leu - Ser - Leu - His - Arg - Thr - Pro - Ala - Gly - Asn - Phe - Val - Thr - Leu - Glu - Glu - Gly - Lys - Gly - Asp - Leu

Species

Human

Host

Rabbit

Reconstitution

Lyophilized antiserum are stable for twelve months from date of receipt when stored at -20°C to -70°C. For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Upon reconstitution, the antibody can be stored at 2Please click here or click the reference tab on the top to see more articles that use this product.