Endothelin-1 (ET-1) (145-162)-Amide, Prepro (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample mouse & rat kidney tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control Pre-immuno serum
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Endothelin-1, prepro (145-162)(Human) Serum (Catalog No.:H-023-05)
Optimal Dilution 1:500 (over night at 40C)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog #

H-023-05

Standard Size

100 µl

Sequence

Gly - Lys - Asp - Cys - Ser - Lys - Leu - Gly - Lys - Lys - Cys - Ile - Tyr - Gln - Gln - Leu - Val - Arg - NH2

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use. For the antiserum, add additional buffer.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of rabbit anti-Endothelin-1 (145-162) (Human) serum in the lyophilized form.

Recommended Dilution Factor

Direct ELISA: 1:3200
Immonohistochemistry (ABC) 1:500

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