GLP-1 (7-36)-Amide (Human, Rat, Mouse, Porcine, Bovine, Canine, Ovine)

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Protocol for Immunohistochemistry
Tissue Sample	Human pancreas tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-GLP-1 (7-36)-NH2 Antiserum (Catalog No.: H-028-11)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-028-11

Family

GLP-1

Standard Size

50 µl

Sequence

His - Ala - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Val - Ser - Ser - Tyr - Leu - Glu - Gly - Gln - Ala - Ala - Lys - Glu - Phe - Ile - Ala - Trp - Leu - Val - Lys - Gly - Arg - NH2

Species

Human, Rat, Porcine, Mouse, Bovine, Ovine, Canine

Host

Rabbit

Immunogen

Glucagon-Like-Peptide-1 (7-36)-Amide

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

Each vial contains 50µl of rabbit Anti-Glucagon-Like Peptide-1 (7-36) Amide (GLP-1) [Prepro-Glucagon (78-107) Amide] (Human) serum in the lyophilized form. Reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum. For diluted antiserum add additional buffer.

Cross Reactivity

*Peptide*	*% Crossreactivity*
GLP-1 (7-36) (Human, Rat, Mouse)	100
GLP-1 (7-37) (Human, Rat, Mouse)	0.4
GLP-2 (Human)	0
Glucagon (Human)	0
Insulin (Human)	0
Secretin (Porcine)	0
GIP (Human)	0
VIP (Human, Porcine, Rat)	0

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