PK2L (48-68) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample Rat and mouse brain tissue
Fixative 10% Formalin
Negative control No primary antibody
Pretreatment Target retrieval 25 min (Steam)
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-PK 2 L (48-68) (Human) Serum (Catalog No.:H-002-78)
Optimal Dilution 1:200 (overnight at cold room)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog #

H-002-78

Standard Size

100 µl

Sequence

Asn - Asn - Phe - Gly - Asn - Gly - Arg - Gln - Glu - Arg - Arg - Lys - Arg - Lys - Arg - Ser - Lys - Arg - Lys - Lys - Glu

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use. Reconstituted antibody can also be aliquotted (5~10ul) and stored frozen at