Angiotensin I (1-7) / Angiotensin II (1-7) (Human, Rat, Mouse, Canine)

多肽

标记多肽

多肽激素文库

抗体

免疫组化抗体
纯化免疫球蛋白

抗体标记

免疫试剂盒

生物标志物阵列

Angiotensin I (1-7) / Angiotensin II (1-7) (Human, Rat, Mouse, Canine) - Antibody for Immunohistochemistry

Catalog#	Standard size	Price	Order
H-002-24	50 µl	$450.00

Image

Protocol for Immunohistochemistry:
Tissue Sample	Rat & Mouse Heart
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Angiotensin II (1-7) (Human) Antiserum (Catalog No.:H-002-24)
Optimal Dilution	1:100
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-24

Standard Size

50 µl

Sequence

Asp - Arg - Val - Tyr - Ile - His - Pro

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit anti- Angiotensin II (1-7) (Human) serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

Alphabet_A_

Please click here or click the reference tab on the top to see more articles that use this product.