Angiotensin II (Human, Rat, Mouse, Canine) - Antibody for Immunohistochemistry

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Angiotensin II (Human, Rat, Mouse, Canine) - Antibody for Immunohistochemistry

Catalog#	Standard size	Price	Order
H-002-12	50 µl	$175.00

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Protocol for Immunohistochemistry:
Tissue Sample	Rat Kidney
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Pronase
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Angiotensin II Antiserum (Catalog No.:H-002-12)
Optimal Dilution	1:2000 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-12

Standard Size

50 µl

Sequence

Asp - Arg - Val - Tyr - Ile - His - Pro - Phe

Species

Human, Rat, Mouse, Porcine, Canine

Specific Specie

Guinea Pig, Rabbit

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit anti- Angiotensin II (Human) serum in the lyophilized form.

Recommended Dilution Factor

ABC: 1:100, 1:200
Western Blot: 1:200
Immunocytochemistry- 1:200 (Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3301-11.)

Cross Reactivity

*Peptide*	*%Cross-reactivity*
Angiotensin II (Human)	100
Angiotensin III (Human)	100
Angiotensin II (1-4) (Human)	0.1
Angiotensin II (5-8) (Human)	0.001
Pre-Angiotensin (1-14)	1
Angiotensin I (Human)	1.7
ACTH (Human)	0
Endothelin-1 (Human, Porcine, Rat)	0
[Arg8]-Vasopressin	0

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