Adiponectin Precursor [Cys0] (222-244) C-terminal (Human)

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Protocol for Immunohistochemistry:
Tissue Sample Rat liver & visceral white fat tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Anti-Adiponectin, (222-244), Cys⁰ (Human) serum (H-028-51)
Optimal Dilution 1: 50-100 (for liver), 1:500~1000 (overnight hour at 4 ⁰C for fat)
Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
Amplification ABC (Vector)
Detection system HRP
Substrate DAB (Sigma)
Counterstained Hematoxylin

Catalog #

H-028-51

Standard Size

50 µl

Sequence

Asn - Gly - Leu - Tyr - Ala - Asp - Asn - Asp - Asn - Asp - Ser - Thr - Phe - Thr - Gly - Phe - Leu - Leu - Tyr - His - Asp - Thr - Asn

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-[Cys0]-Adiponectin (222-244) (Human) Serum in the lyophilized form.

Recommended Dilution Factor

Western Blotting: 1: 100-200
IHC: 1: 50-100

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