Bombesin Receptor Subtype-3 (BRS-3) (371-399) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample Rat stomach tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Bombesin Receptor Subtype-3 (371-399) Serum (Catalog No.:H-001-82)
Optimal Dilution 1:50 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog #

H-001-82

Standard Size

100 µl

Sequence

Val - Pro - Gly - Thr - Gly - Ser - Ile - Gln - Met - Ser - Glu - Ile - Ser - Val - Thr - Ser - Phe - Thr - Gly - Cys - Ser - Val - Lys - Gln - Ala - Glu - Asp - Arg - Phe

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100ul of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of rabbit anti- Receptor Subtype-3 (BRS-3) (371-399) (Human) serum in the lyophilized form

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