Protocol for Immunohistochemistry:
Tissue Sample
Rat Lung
Fixative
10% formalin
Embedding
Paraffin
Negative Control
No primary antibody
Pretreatment
Target Retrieval 10 min (micro-wave oven)
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-APJ (357-377) (Rat) Antiserum (Catalog No.:H-001-79)
Optimal Dilution
1:1000 (1 hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Links to publications that use this antibody:
Tiani et al. Apelin signaling modulates splanchnic angiogenesis and portosystemic collateral vessel formation in rats with portal hypertension.J Hepatol. 2009 Feb;50(2):296-305.
Iwanaga et al. Down-regulation of cardiac apelin system in hypertrophied and failing hearts: Possible role of angiotensin II-angiotensin type 1 receptor system. J Mol Cell Cardiol. 2006 Nov;41(5):798-806.
Jia et al. Apelin protects myocardial injury induced by isoproterenol in rats. Regul Pept. 2006 Jan 15;133(1-3):147-54.
Kleinz et al. Immunocytochemical localisation of the apelin receptor, APJ, to human cardiomyocytes, vascular smooth muscle and endothelial cells. Regul Pept. 2005 Mar 30;126(3):233-40.
Zhong et al. Effects of all-trans retinoic acid on orphan receptor APJ signaling in spontaneously hypertensive rats.Cardiovasc Res. 2005 Feb 15;65(3):743-50.
