LGR8 (737-754) [Cys0] (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample Rat hypothalamus
Fixative 10% formalin
Embedding Paraffin
Negative Control No primary antibody
Pretreatment Pronase
Blocking 2% Normal Goat Serum, 3% H₂O₂
Primary Antibody LGR8 (737-754), Cys0, (Human) Antiserum (Catalog No.: H-001-54)
Optimal Dilution 1:100 (1 hour at RT)
Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification ABC (Vector)
Detection System HRP
Substrate DAB (Sigma)
Counterstained Hematoxylin

Catalog #

H-001-54

Standard Size

50 µl

Sequence

Cys - Leu - Gly - Val - Leu - Asn - Lys - Ile - Thr - Leu - Gly - Asp - Ser - Ile - Met - Lys - Pro - Val - Ser

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit anti- LGR8 (737-754) [Cys0] (Human) serum in the lyophilized form.

Recommended Dilution Factor

Immunohistochemistry - 1:100, 1:200, 1:300. Reprod Biol Endocrinol. 2007 Jul 10;5:29.

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Filonzi et al. Relaxin family peptide receptors Rxfp1 and Rxfp2: mapping of the mRNA and protein distribution in the reproductive tract of the male rat.
Reprod Biol Endocrinol. 2007 Jul 10;5:29.

Bennett et al. Relaxin receptors in hepatic stellate cells and cirrhotic liver.
Biochem Pharmacol. 2007 Apr 1;73(7):1033-40.

Bennett et al. Relaxin receptor expression in hepatic stellate cells and in cirrhotic rat liver tissue.
Ann N Y Acad Sci. 2005 May;1041:185-9.