INSL 3 / RLF (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample Human Testis
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Intact
Blocking 3% H₂O₂, 2% Normal Goat Serum
Primary Antibody Rabbit Anti-INSL 3 (Human) (Catalog No.:H-035-27)
Optimal Dilution 1: 1000~2000 (1 hour at room temperature)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification ABC (Vector)
Detection system HRP
Substrate DAB (Sigma)
Counterstained Hematoxylin

Catalog #

H-035-27

Standard Size

50 µl

Sequence

A Chain AAATNPARYCCLSGCTQQDLLTLCPY B Chain PTPEMREKLCGHHFVRALVRVCGGPRWSTEA

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- INSL 3 (Human) Serum in the lyophilized form.

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Foresta C, Strapazzon G, De toni L, et al. Bone mineral density and testicular failure: evidence for a role of vitamin D 25-hydroxylase in human testis.
J Clin Endocrinol Metab. 2011;96(4):E646-52.

Lehmann et al. Dose-dependent alterations in gene expression and testosterone synthesis in the fetal testes of male rats exposed to di (n-butyl) phthalate.
Toxicol Sci. 2004 Sep;81(1):60-8.