Relaxin & Relaxin-Related Peptides

Insulin-like 6 Peptide (INSL 6)

Relaxin Evolution

Antibody Production & Evaluation

Image	Protocol for Immunohistochemistry:   Tissue Sample  Rat testis  Fixative  10% Formalin  Embedding  Paraffin  Negative control  No primary antibody  Pretreatment  Target retrieval 25 min (Steam)  Blocking  3% H2O2, 2% Normal Goat Serum  Primary Antibody  Anti-INSL-6, A Chain (Human) (Human) Antibody (Catalog No.: H-035-28)  Optimal Dilution  1:2000 (1hour at RT)  Secondary Antibody  Goat anti-Rabbit IgG, Biotinylated (Vector) (1:400), 30 min  Amplification  Streptavidin-HRP (1:400), 30 min  Detection system  HRP  Substrate  DAB (Sigma)  Counterstained  Hematoxylin, 30 sec
Catalog #	H-035-28
Standard Size	50 µl
Sequence	Gly - Tyr - Ser - Gly - Lys - Cys - Cys - Leu - Thr - Gly - Cys - Thr - Lys - Glu - Glu - Leu - Ser - Ile - Ala - Cys - Leu - Pro - Tyr - Ile - Asp - Phe
Species	Human
Host	Rabbit
Reconstitution	For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition	Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content	This vial contains 50µl of rabbit anti- INSL6 (RIF-1) A-Chain (Human) serum in the lyophilized form.

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