INSL 3 C-Peptide (Human) - Antibody for Immunohistochemistry
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| Catalog# |
Standard size |
Price |
Order |
| H-035-48 |
100 µl |
$450.00 |
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| Protocol for Immunohistochemistry: | | Tissue Sample | Rat brain and testis tissue | | Fixative | 10% Formalin | | Embedding | Paraffin | | Negative control | No primary antibody | | Pretreatment | Target retrieval 25 min (Steam) | | Blocking | 2% Normal Goat Serum | | Primary Antibody | Rabbit Anti-Insulin-like 3 C Peptide/Insulin-like 3 Prepro (58-105) (Human) Serum (Catalog No: H-035-48) | | Optimal Dilution | 1:50-200 (1hour at RT) | | Secondary Antibody | Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min) | | Amplification | ABC (Vector) (1:400, 30 min) | | Detection system | HRP | | Substrate | DAB (Sigma), 3 min | | Counterstained | Hematoxylin, 30 Sec |
| | Catalog # | H-035-48 | | Standard Size | 100 µl | | Sequence | Pro - Ala - Ala - Gly - Gly - Asp - Arg - Glu - Leu - Leu - Gln - Trp - Leu - Glu - Arg - Arg - His - Leu - Leu - His - Gly - Leu - Val - Ala - Asp - Ser - Asn - Leu - Thr - Leu - Gly - Pro - Gly - Leu - Gln - Pro - Leu - Pro - Gln - Thr - Ser - His - His - His - Arg - His - His - Arg | | Species | Human | | Host | Rabbit | | Reconstitution | For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at
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