INSL 3 C-Peptide (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample Rat brain and testis tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Target retrieval 25 min (Steam)
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Insulin-like 3 C Peptide/Insulin-like 3 Prepro (58-105) (Human) Serum (Catalog No: H-035-48)
Optimal Dilution 1:50-200 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog #

H-035-48

Standard Size

100 µl

Sequence

Pro - Ala - Ala - Gly - Gly - Asp - Arg - Glu - Leu - Leu - Gln - Trp - Leu - Glu - Arg - Arg - His - Leu - Leu - His - Gly - Leu - Val - Ala - Asp - Ser - Asn - Leu - Thr - Leu - Gly - Pro - Gly - Leu - Gln - Pro - Leu - Pro - Gln - Thr - Ser - His - His - His - Arg - His - His - Arg

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at