Neuromedin S (Rat) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample Rat brain tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody (pre-immuno serum)
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-Neuromedin S (Rat) Antiserum (Catalog No.:H-045-87)
Optimal Dilution 1:500 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog #

H-045-87

Standard Size

100 µl

Sequence

Leu - Pro - Arg - Leu - Leu - His - Thr - Asp - Ser - Arg - Met - Ala - Thr - Ile - Asp - Phe - Pro - Lys - Lys - Asp - Pro - Thr - Thr - Ser - Leu - Gly - Arg - Pro - Phe - Phe - Leu - Phe - Arg - Pro - Arg - Asn - NH2

Species

Rat

Host

Rabbit

Reconstitution

Lyophilized antiserum are stable for twelve months from date of receipt when stored at -20