KiSS-1 (112-121) Amide / Kisspeptin-10 / Metastin (45-54) Amide (Human)

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KiSS-1 (112-121) Amide / Kisspeptin-10 / Metastin (45-54) Amide (Human) - Antibody for Immunohistochemistry

Catalog#	Standard size	Price	Order
H-048-56	50 µl	$450.00

Image

Western Blot Analysis of Kiss-1 / Prepro-metastin in rat brain tissue
Tissue Sample	Rat brain tissue homogenate
Electrophoresis	10% SDS-PAGE gel
Negative control	No primary antibody
Blocking	1% BSA in TBST or 5% dry milk in TBST
Primary Antibody	Rabbit Anti-Metastin (45-54) (Human) Antiserum (Catalog No.:H-048-56)
Optimal Dilution	1:500-~1000 (4 hour at RT, or overnight at 4^oC)
Secondary Antibody	Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system	HRP
Substrate	SuperSignal Chemiluminescent Substrate

Rat brain and spinal cord tissue was stained by this antibody using the following protocol [Neurosci Lett. 2003 Jan 2;335(3):197-201.]

Protocol for Immunohistochemistry:
Tissue Sample Rat medulla oblongata and spinal cord
Fixative 4%paraformaldehyde/ 4%picric acid
Embedding Paraffin
Negative control No primary antibody or primary antibody pre-absorbed with the metastin (45-54)-NH2 peptide (10µg/ml) overnight
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Metastin (45-54) (Human) Antibody (Code:H-048-56)
Optimal Dilution 1: 1500
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:150)
Amplification ABC (Vector)
Detection system HRP
Substrate DAB (Sigma)
Counterstained No

Catalog #

H-048-56

Standard Size

50 µl

Sequence

Tyr - Asn - Trp - Asn - Ser - Phe - Gly - Leu - Arg - Phe - NH2

Species

Human

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- Metastin (45-54)-NH2 (Human) Serum in the lyophilized form. The HPLC purified standard peptide, Metastin (45-54)-NH2 (Human) can be detected with this antiserum (1:16000, 0.062µl/ml) in ELISA.

Recommended Dilution Factor

Immunohistochemistry - 1:1000. Neurosci Lett. 2003 Jan 2;335(3):197-201.
Immunohistochemistry - 1:1000. Neurosci Lett. 2007 Jun 8;420(1):12-7.
1: 500 ~ 1000 (western blot)
1: 6400 (indirect ELISA)
1: 500~ 1000 (Immunohistochemistry-ABC)

Cross Reactivity

This antibody is crossreactivity with human , rat and mouse KiSS-1 (112-121)-Amide / Kisspeptin-10 / Metastin (45-54)-NH2 peptide at 100%. It has been tested in rat or mouse brain tissue.

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