Apelin-36 (Rat, Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human placenta tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Apelin-36 (Rat, Mouse) Antiserum (Catalog No.:H-057-28)
Optimal Dilution	1:200 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma)
Counterstained	Hematoxylin

Catalog #

H-057-28

Standard Size

50 µl

Sequence

Leu - Val - Lys - Pro - Arg - Thr - Ser - Arg - Thr - Gly - Pro - Gly - Ala - Trp - Gln - Gly - Gly - Arg - Arg - Lys - Phe - Arg - Arg - Gln - Arg - Pro - Arg - Leu - Ser - His - Lys - Gly - Pro - Met - Pro - Phe

Species

Rat, Mouse

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Tiani et al. Apelin signaling modulates splanchnic angiogenesis and portosystemic collateral vessel formation in rats with portal hypertension.
J Hepatol. 2009 Feb;50(2):296-305.

Susaki et al. Apelin cells in the rat stomach.
Regul Pept. 2005 Jul 15;129(1-3):37-41.