Neuropeptide S, Prepro (23-67) (Mouse) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry
Tissue Sample	Rat and Mouse Brain Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Target Retrieval 25 min (Steam)
Blocking	2% Normal Goat Serum, 3%H₂O₂
Primary Antibody	Rabbit anti-Neuropeptide S, Prepro (23-67) Serum (Catalog No.: H-005-99)
Optimal Dilution	1:500 (1 hour at RT )
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification	ABC (Vector)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin

Catalog #

H-005-99

Family

Neuropeptide S

Standard Size

100 µl

Sequence

Cys - Tyr - Pro - Val - Leu - Ser - Ser - Lys - Val - Pro - Gly - Lys - Pro - Asp - Tyr - Phe - Leu - Ile - Leu - Leu - Ser - Ser - Cys - Pro - Ala - Arg - Leu - Glu - Gly - Ser - Asp - Arg - Leu - Ala - Phe - Leu - Lys - Pro - Ile - Leu - Glu - Lys - Thr - Ser - Met Disulfide bridge Cys23 - Cys45

Species

Mouse

Host

Rabbit

Immunogen

Neuropeptide S, Prepro (23-67) (Mouse)

Reconstitution

100 ul

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry (Rat Brain) - 1:200
Immunohistochemistry (Mouse Brain) - 1:500

Alphabet_N_