Adiponectin Precursor [Cys0] (223-244) (Rat, Mouse) - Antibody for Immunohistochemistry

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Protocol for Western Blot:
Sample	Rat serum, plasma, visceral fat tissue
Electrophoresis	10% SDS-PAGE gel, reducing condition
Denatured sample	100'C for 5 min
Positive control	Adiponectin (223-244), Cys0, (Rat) peptide
Blocking	1% BSA in TBST
Primary Antibody	Rabbit Anti-Adiponectin (222-244) (Rat) Antiserum (Catalog No.:H-028-54, Lot No.: PB105-2)
Optimal Dilution	1:500 (overnight at cold room)
Secondary Antibody	Rabbit anti-Mouse IgG, HRP conjugated (1: 12500)
Substrate	ECL
Positive Band	31 ~32 KDa

Protocol for Immunohistochemistry:
Tissue Sample	Rat visceral white fat tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Adiponectin (223-244) (Rat) Antiserum (Catalog No.:H-028-54, Lot No.: PB105-2)
Optimal Dilution	1:500~1000 (overnight hour at 4 ⁰C)
Secondary Antibody	Goat anti-Rabbit IgG, HRP conjugated
Amplification	ABC (Vector)
Detection system	HRP
Substrate	DAB (Sigma)
Counterstained	Hematoxylin

Catalog #

H-028-54A

Standard Size

100 µl

Species

Rat

Host

Rabbit

Reconstitution

Reconstitute with 100 ul or 200 ul distill water or PBS

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

1: 3200 ( indirect ELISA )
1: 500 ~ 1000 (Western blot analysis)
1: 500 ~ 1000 (Immunohistochemistry)

Cross Reactivity

This antibody is crossreactivity with rat adiponectin (223-244). It also tested in rat white fat tissue in western blot analysis and immunohistmochemistry.

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