Defensin 1, beta (Human) (synthetic) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat & Mouse lung, Human lung cancer tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No Primary Antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Beta-Defensin I (H) Antiserum (Catalog No.: H-072-53)
Optimal Dilution	1:500 , 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-072-53

Standard Size

100 µl

Sequence

Asp - His - Tyr - Asn - Cys - Val - Ser - Ser - Gly - Gly - Gln - Cys - Lys - Tyr - Ser - Ala - Cys - Pro - Ile - Phe - Thr - Lys - Ile - Gln - Gly - Thr - Cys - Tyr - Arg - Gly - Lys - Ala - Lys - Cys - Cys - Lys Disulfide bridges between Cys1 & Cys5, Cys2 & Cys4 and Cys3 & Cys6

Species

Human

Host

Rabbit

Immunogen

Beacon (47-73)

Reconstitution

Rehydrate the lypholized antiserum with 100µl deionized water, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of Rabbit Anti- β-Defensin 1 (Human) Serum in the lyophilized form.

Recommended Dilution Factor

Indirect ELISA 1: 6400
Immunohistochemistry 1: 250~ 500

Cross Reactivity

This antiserum exhibits 100% cross-reactivity with beacon (47-73) of Psammomys obesus, and does not cross-react with following peptides: angiotensin I (human), cholecystokinin octapeptide (26-33 non-sulfated form), neurotensin, galanin (porcine), motilin (porcine), g3-MSH, somatostatin-28, arginine-vasopressin, calcitonin (rat), neuropeptide Y (human, rat), or glucagons-like peptide-1 (7-37).

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