Defensin 3, beta (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat & Mouse lung tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Beta-Defensin 3 (H) Antiserum (Catalog No.: H-072-42)
Optimal Dilution	1:500 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-072-42

Standard Size

100 µl

Sequence

Gly - Ile - Ile - Asn - Thr - Leu - Gln - Lys - Tyr - Tyr - Cys - Arg - Val - Arg - Gly - Gly - Arg - Cys - Ala - Val - Leu - Ser - Cys - Leu - Pro - Lys - Glu - Glu - Gln - Ile - Gly - Lys - Cys - Ser - Thr - Arg - Gly - Arg - Lys - Cys - Cys - Arg - Arg - Lys - Lys
Disulfide bridges between Cys1 & Cys5, Cys2 & Cys4 and Cys3 & Cys6

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 100ml of distilled water for the equivalent of undiluted antiserum, immediately before use. For the antiserum, add additional buffer. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 100µl of Rabbit Anti- β-Defensin 3 (Human) (Synthetic) Serum in the lyophilized form.

Recommended Dilution Factor

Indirect ELISA 1: 6400
PAP or ABC 1: 250~500

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