MCH-2R [Cys0] (237-251) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample Human ventral lateral hypothalamus
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Target retrieval 25 min (Steam)
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-MCH-R2 (237-251), Cys⁰, (Human) Serum (Catalog No.:H-071-20)
Optimal Dilution 1:2000 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec

Catalog #

H-071-20

Standard Size

50 µl

Sequence

Cys - Asn - Pro - Ser - Val - Pro - Lys - Gln - Arg - Val - Met - Lys - Leu - Thr - Lys

Species

Human

Host

Rabbit

Immunogen

Melanin-Concentrating Hormone Receptor 2 (MCH-2R) (237-251) (Human)

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-MCH-2R (237-251) (human) Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

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