PYY (Rat, Mouse, Porcine, Canine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Rat colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (Porcine, Rat) Antiserum (Catalog No.:H-059-03)
Optimal Dilution	1:200, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-059-03

Standard Size

100 µl

Sequence

Tyr - Pro - Ala - Lys - Pro - Glu - Ala - Pro - Gly - Glu - Asp - Ala - Ser - Pro - Glu - Glu - Leu - Ser - Arg - Tyr - Tyr - Ala - Ser - Leu - Arg - His - Tyr - Leu - Asn - Leu - Val - Thr - Arg - Gln - Arg - Tyr - NH2

Species

Rat, Porcine

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- Peptide YY (Porcine, Rat) Serum in the lyophilized form.

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Mihwa Choi, Jun Ozeki, Masami Hashizume, Shigeaki Kato, Hisamitsu Ishihara, and Makoto Makishima. Vitamin D Receptor Activation Induces Peptide YY Transcription in Pancreatic Islets.
Endocrinology. 2012 Nov;153(11):5188-99. doi: 10.1210/en.2012-1396. Epub 2012 Sep 7.

Bezencon et al. Taste-Signaling Proteins Are Coexpressed in Solitary Intestinal Epithelial Cells
Chem Senses. 2007 Jan;32(1):41-9.