Hypothetical Protein XP_294524 (154-196) [pGlu154] Amide / QRFP-43 (Human)

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Western Blot Analysis of Prepro-QRFP in rat brain tissue
Tissue Sample	Rat brain tissue homogenate
Electrophoresis	10% SDS-PAGE gel
Negative control	No primary antibody
Blocking	1% BSA in TBST or 5% dry milk in TBST
Primary Antibody	Rabbit Anti-QRFP-43 (Human) Antiserum (Catalog No.:H-048-78)
Optimal Dilution	1:500-~1000 (4 hour at RT, or overnight at 4' C)
Secondary Antibody	Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system	HRP
Substrate	SuperSignal Chemiluminescent Substrate

Protocol for Immunohistochemistry:
Tissue Sample Rat or Mouse brain Tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-QRFP-43 (Human) Antiserum (Catalog No.:H-048-78)
Optimal Dilution 1:100 -200 (1 hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (sigma), 30min
Counterstained Hematoxylin, 30Sec

Catalog #

H-048-78

Standard Size

50 µl

Sequence

pGlu - Asp - Glu - Gly - Ser - Glu - Ala - Thr - Gly - Phe - Leu - Pro - Ala - Ala - Gly - Glu - Lys - Thr - Ser - Gly - Pro - Leu - Gly - Asn - Leu - Ala - Glu - Glu - Leu - Asn - Gly - Tyr - Ser - Arg - Lys - Lys - Gly - Gly - Phe - Ser - Phe - Arg - Phe - NH2

Species

Human

Specific Specie

This antibody is crossreactivity with human , rat and mouse QRFP-43 peptide at 100%. It has been tested in rat or mouse brain tissue.

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- QRFP-43-Amide (Human) Serum in the lyophilized form.

Recommended Dilution Factor

1: 500 ~ 1000 (western blot)
1: 6400 (indirect ELISA)
1: 100 ~ 200 (Immunohistochemistry-ABC)

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